Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,043,829	G→A	E106E (GAG→GAA)	yodA →	conserved metal‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,043,829	0	G	A	100.0%	55.7 / NA	32	E106E (GAG→GAA)	yodA	conserved metal‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/32);  total (0/32)

GCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATG  >  W3110S.gb/2043815‑2043875               |                                               gcgaCAGATATCGAAATGATTGGCATTGAGGACTGCATTGTCGAATTCCATAGAAATAATg  <  1:15993/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:74771/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:54850/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:55144/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:59800/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:62404/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:69556/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:71076/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:73703/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:74423/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:5459/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:78149/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:80844/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:81183/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:85622/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:87802/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:9695/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:11119/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:50560/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:50310/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:42229/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:40914/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:36879/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:31733/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:29662/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:25951/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:25763/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:22230/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:21849/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:20989/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:17125/58‑1 (MQ=255) gcgaCAGATATCGAAATGATTGGCATTGAGGACGGCATTGTCGAATTCCATAGAAATAATg  <  1:12414/58‑1 (MQ=255)               |                                               GCAACAGATATCGAGATGATTGGCATTGAGGACGGCATTGTTGAATTCCATAGAAATAATG  >  W3110S.gb/2043815‑2043875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑