Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,081,721	C→T	A336A (GCG→GCA)	yeeA ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,081,721	0	C	T	100.0%	169.3 / NA	90	A336A (GCG→GCA)	yeeA	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (90/0);  total (90/0)

GCGCAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGCGCCGTTTCCATGTTCA  >  W3110S.gb/2081676‑2081737                                              |                 gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCa        >  1:42713/1‑56 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTc   >  1:14029/1‑61 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTc   >  1:6131/1‑61 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:620/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:69623/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:68576/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:67910/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:66707/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:6530/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:64027/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:63769/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:632/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:6259/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:62489/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:74732/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:59040/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:58448/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:5832/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:58058/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:57051/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:57046/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:55019/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:54566/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:54371/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:82926/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:88591/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:88575/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:87406/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:87036/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:85428/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:85340/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:85022/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:83821/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:83677/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:83606/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:51603/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:81950/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:803/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:80057/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:79016/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:77786/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:77772/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:77525/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:77230/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:75998/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:75022/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:18398/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:31867/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:31804/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:2386/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:22702/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:21752/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:21364/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:20324/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:19436/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:1922/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:19193/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:32669/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:17673/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:17622/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:17440/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:16731/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:16670/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:15650/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:14148/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:12828/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:12457/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:11832/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:42769/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:51494/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:51373/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:49598/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:48129/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:46783/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:44887/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:44325/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:43813/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:43384/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:10648/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:4172/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:40620/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:40467/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:39507/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:38117/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:37138/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:37122/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:36843/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:36086/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGTTCa  >  1:33415/1‑62 (MQ=255) gcgcAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGTGCCGTTTCCATGATCa  >  1:56756/1‑62 (MQ=255)                                              |                 GCGCAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGCGCCGTTTCCATGTTCA  >  W3110S.gb/2081676‑2081737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑