Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,100,337	G→A	I34I (ATC→ATT)	cld ←	regulator of length of O‑antigen component of lipopolysaccharide chains

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,100,337	0	G	A	100.0%	118.2 / NA	66	I34I (ATC→ATT)	cld	regulator of length of O‑antigen component of lipopolysaccharide chains
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (66/0);  total (66/0)

GGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTA  >  W3110S.gb/2100309‑2100371                             |                                   gggCAATAGCCACAATGACGGAAATGATAATTGTCATCt                          >  1:71628/1‑39 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTa     >  1:1051/1‑60 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTa     >  1:76174/1‑60 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:53480/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:56175/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:57839/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:58623/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:58777/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:59637/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:52996/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:6018/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:6069/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:61289/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:62193/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:68798/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:62305/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:64071/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:68398/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:68731/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:51227/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:70201/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:72260/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:72392/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:74327/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:7447/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:79475/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:80624/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:81281/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:82147/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:8276/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:84203/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:85124/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:87735/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:30589/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:12325/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:13567/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:15039/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:1537/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:16216/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:16231/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:17400/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:20063/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:24910/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:26354/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:27126/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:27302/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:27521/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:29041/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:50516/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:39521/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:46952/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:45137/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:43956/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:43856/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:42705/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:40389/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:39608/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:33055/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:37191/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:3662/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:36096/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:3551/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:34131/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAg    >  1:33650/1‑61 (MQ=255) gggCAATAGCCACAATGACGGAAATGATAATTGTCATCTAGCCACGCCACAACTGc         >  1:74322/1‑56 (MQ=38) gggCAATAGCCA‑AATGACGGAAATGATAATTGTCATCTTGCCACGCCACAACTGCACTAGTa  >  1:19950/1‑62 (MQ=255)                             |                                   GGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTGCACTAGTA  >  W3110S.gb/2100309‑2100371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑