Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,124,781	C→T	E112E (GAG→GAA)	cpsG ←	phosphomannomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,124,781	0	C	T	100.0%	74.2 / NA	42	E112E (GAG→GAA)	cpsG	phosphomannomutase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (42/0);  total (42/0)

CGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCC  >  W3110S.gb/2124769‑2124811             |                               cGGGCGAGCCCCTTCGCGCCCCAGTTTCATGCCGTTGTAATcc  >  1:49532/1‑43 (MQ=19) cGGGCGAGCCCCTTCGCGCCCCAGTTTCATGCCGTTGTAATcc  >  1:3897/1‑43 (MQ=19) cGGGCGAGCCCCTTCGCGCACCAGTTTCGTGCCGTTGTAATcc  >  1:31135/1‑43 (MQ=16) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:6145/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:55668/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:63432/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:6370/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:64743/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:67874/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:727/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:72798/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:73152/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:7593/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:78834/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:79049/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:79175/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:80024/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:81956/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:82308/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:82936/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:8295/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:85538/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:9872/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:4115/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:15272/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:16773/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:18574/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:20371/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:2207/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:24514/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:29684/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:35679/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:37940/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:58020/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:42051/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:42528/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:42998/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:45300/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:46202/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:48723/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:52099/1‑43 (MQ=25) cGGGCGAGCCCCTTCGCGCACCAGTTTCATGCCGTTGTAATcc  >  1:10230/1‑43 (MQ=25)             |                               CGGGCGAGCCCCCTCGCGAACCAGCTTCATGCCGTTATAATCC  >  W3110S.gb/2124769‑2124811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑