Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,141,591 G→A R518H (CGT→CAT)  yegH → fused predicted membrane proteins

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,141,5910GA99.3% 243.2 / NA 136R518H (CGT→CAT) yegHfused predicted membrane proteins
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (135/0);  total (136/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01

Only 100 of 136 total aligned reads displayed.

AACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATG  >  W3110S.gb/2141538‑2141599
                                                     |        
aaCGTTGCAGGTGGAAGGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:35597/1‑62 (MQ=37)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGGAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:78704/1‑62 (MQ=255)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGaag  >  1:26437/1‑60 (MQ=37)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGAt   >  1:12257/1‑61 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGAt   >  1:32639/1‑61 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGAt   >  1:36556/1‑61 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGAt   >  1:27454/1‑61 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:69498/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:7263/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:68824/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:67880/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:67774/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:67204/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:66375/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:65943/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:65659/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:64998/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:64566/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:6416/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:6382/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:62012/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:60806/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:60539/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:59717/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:58414/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:56491/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:55479/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:53790/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:53393/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:79719/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:88315/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:86556/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:86301/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:85747/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:85019/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:84429/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:83233/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:82983/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:8234/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:81275/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:80766/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:70559/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:78312/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:77798/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:76432/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:76291/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:7623/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:74506/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:74480/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:7325/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:49530/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:72445/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:72070/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:20686/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:33754/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:31844/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:31628/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:30017/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:29122/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:28381/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:27831/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:24069/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:23162/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:22189/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:21419/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:3410/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:19842/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:18044/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:17517/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:16860/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:1637/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:15975/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:1377/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:12236/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:10758/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:10172/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:4185/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:49666/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:1003/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:48166/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:48156/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:44972/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:44287/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:42686/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:42391/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:41990/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:50063/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:41341/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:39964/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:37835/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:37286/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:36660/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:35405/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:35252/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:34477/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:34337/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:34274/1‑62 (MQ=38)
aaCGTTGCAGGTGGAAAGCCATCGCGTGCAGAAGGTACAGATTATTCCGCTGCAAAAGGATg  >  1:37763/1‑62 (MQ=37)
aaCGTTGCAGGTGGAAAGCCATAGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGAt   >  1:45840/1‑61 (MQ=37)
aaCGTTGCAGGTGGAAAGCCAACGCGTGCAGAAGGTACAGATTATTCCGCTGCATAAGGATg  >  1:88281/1‑62 (MQ=37)
                                                     |        
AACGTTGCAGGTAGAAAGCCATCGCGTGCAGAAGGTGCAGATTATACCGCTGCGTAAGGATG  >  W3110S.gb/2141538‑2141599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑