Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,192,489	A→C	F586V (TTT→GTT)	yehB ←	predicted outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,192,489	0	A	C	100.0%	115.7 / NA	60	F586V (TTT→GTT)	yehB	predicted outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (60/0);  total (60/0)

TTCCTGATAATCCCGTATTATTTGAGGCAAACCCCTGATCATCAAACGTCGTTGAGTTA  >  W3110S.gb/2192459‑2192517                               |                             ttCCTGCTAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:27861/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGatcat                   >  1:35458/1‑42 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGa                       >  1:15984/1‑38 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:65965/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:49935/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:50389/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:50877/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:51958/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:53059/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:53618/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:54877/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:592/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:60128/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:62942/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:63192/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:63708/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:48902/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:66948/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:68639/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:70019/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:72163/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:72782/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:73448/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:75113/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:76945/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:78791/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:78841/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:79714/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:87005/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:87010/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:21002/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:13596/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:1372/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:13737/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:15477/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:16294/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:16630/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:18289/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:18317/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:1843/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:20431/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:20558/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:12473/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:2314/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:28279/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:29458/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:31462/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:31668/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:31962/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:32057/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:33001/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:36740/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:40070/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:40492/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:42922/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:46468/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAACGGCGTTGAGTTa  >  1:80553/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGGCAACCCCCTGATCATCAAAAGTCGTTGAGTTa  >  1:69175/1‑59 (MQ=255) ttCCTGATAATCCCGTATTATTTGAGACAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:16890/1‑59 (MQ=255) ttCCTGATAATCCCGAATTATTTGAGGCAACCCCCTGATCATCAAACGTCGTTGAGTTa  >  1:51223/1‑59 (MQ=255)                               |                             TTCCTGATAATCCCGTATTATTTGAGGCAAACCCCTGATCATCAAACGTCGTTGAGTTA  >  W3110S.gb/2192459‑2192517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑