Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,207,155	G→T	V1181V (GTG→GTT)	yehI →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,207,155	0	G	T	100.0%	65.1 / NA	37	V1181V (GTG→GTT)	yehI	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (37/0);  total (37/0)

GCTTCCGTTCTCTTCGAGTGTACCGTGACAATAAATCCGTCACTTTTGCCG  >  W3110S.gb/2207136‑2207186                    |                                gCTTCCGTTCTCTTTGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:39953/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATGCGTCACTTTTGCCg  >  1:80387/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGc    >  1:40498/1‑49 (MQ=39) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:74578/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:57271/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:60583/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:60848/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:61799/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:65023/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:65160/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:67981/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:52518/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:76911/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:77536/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:82639/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:83442/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:85727/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:86813/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:88298/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:30933/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:16763/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:21506/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:21959/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:25037/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:25264/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:25887/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:29065/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:29895/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:56986/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:34478/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:34737/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:34748/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:37670/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:38191/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:46946/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTGACAATAAATCCGTCACTTTTGCCg  >  1:16199/1‑51 (MQ=255) gCTTCCGTTCTCTTCGGGTTTACCGTAACAATAAATCCGTCACTTTTGCCg  >  1:67189/1‑51 (MQ=255)                    |                                GCTTCCGTTCTCTTCGAGTGTACCGTGACAATAAATCCGTCACTTTTGCCG  >  W3110S.gb/2207136‑2207186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑