Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,264,022	G→A	V241V (GTC→GTT)	fruA ←	fused fructose‑specific PTS enzyme IIB'BC components

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,264,022	0	G	A	100.0%	75.1 / NA	42	V241V (GTC→GTT)	fruA	fused fructose‑specific PTS enzyme IIB'BC components
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/42);  total (0/42)

CCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAA  >  W3110S.gb/2263981‑2264040                                          |                   tcaaaagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:74605/59‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:68786/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:47036/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:12531/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:4770/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:52229/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:55785/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:57856/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:59152/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:63806/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:64642/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:47232/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:70132/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:74538/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:80641/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:83930/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:85951/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:86180/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:87476/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:37570/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:1313/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:13839/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:183/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:19915/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:22765/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:26580/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:27515/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:32138/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:32686/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:46163/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:40345/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:40468/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:40759/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:40978/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:43298/56‑1 (MQ=255)     aagcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:44141/56‑1 (MQ=255)     aagcaaaagAAA‑‑GCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:78688/54‑1 (MQ=38)      agcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:78965/55‑1 (MQ=255)      agcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:43126/55‑1 (MQ=255)      agcaaaagAAAGCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:45782/55‑1 (MQ=255)            agaaagCGCGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:64500/49‑1 (MQ=255)                 gcgcGATACACAGACCACCTGCAACAACCATCGGCAGCATGTaa  <  1:54275/44‑1 (MQ=255)                                          |                   CCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAA  >  W3110S.gb/2263981‑2264040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑