Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	247,254	C→T	N181N (AAC→AAT)	yafL →	predicted lipoprotein and C40 family peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	247,254	0	C	T	100.0%	135.2 / NA	73	N181N (AAC→AAT)	yafL	predicted lipoprotein and C40 family peptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (73/0);  total (73/0)

CACGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCG  >  W3110S.gb/247206‑247268                                                 |               cACGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGcc   >  1:26520/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCTGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:67477/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCACCGATTGTGGCGAATAACGCCCTGCGCCg  >  1:64931/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGCCCTGCGCCg  >  1:27280/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGAc          >  1:51524/1‑54 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:54316/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:55712/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:56618/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:57892/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:5920/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:59241/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:61133/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:61569/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:61731/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:54089/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:6197/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:53256/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:52313/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:63125/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:63690/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:65752/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:79855/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:9614/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:87671/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:8660/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:85237/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:84295/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:83613/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:83421/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:82718/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:64887/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:77290/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:75821/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:71229/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:68009/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:67592/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:65900/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:49790/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:19994/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:30714/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:29775/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:26739/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:26647/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:25868/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:22684/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:2164/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:21484/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:31061/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:19889/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:16995/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:15985/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:13226/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:131/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:1143/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:10907/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:10902/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:31407/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:47551/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:47198/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:46663/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:45219/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:44332/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:42991/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:42282/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:42001/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:4190/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:40330/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:34594/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:3300/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:32533/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:31959/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCg  >  1:49855/1‑62 (MQ=255)  aCGGCCAATGAGATGTACCACTACCGTCGGGCAACGATTGTGGCGAATAAAGACCTGCGCCg  >  1:1046/1‑62 (MQ=255)                                                 |               CACGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCG  >  W3110S.gb/247206‑247268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑