Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,267,108 C→T intergenic (‑279/‑89) fruB ← / → setB fused fructose‑specific PTS enzyme IIA component and HPr component/lactose/glucose efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,267,1080CT100.0% 50.5 / NA 30intergenic (‑279/‑89)fruB/setBfused fructose‑specific PTS enzyme IIA component and HPr component/lactose/glucose efflux system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/30);  total (0/30)

CAATCAGCAACAGTGAAATGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTT  >  W3110S.gb/2267079‑2267141
                             |                                 
cAATCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGtt   <  1:32641/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:15267/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:8722/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:86107/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:83724/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:82162/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:78693/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:75870/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:74504/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:69154/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:68353/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:67263/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:65890/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:65039/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:627/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:61030/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:56641/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:49573/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:48025/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:4726/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:34880/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:34868/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:26216/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:24946/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:21891/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:20722/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:19701/62‑1 (MQ=255)
 aaTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:14062/62‑1 (MQ=255)
  aTCAGCAACAGTGAAATGGCATTTTGATTATTCGTGGCGCTTTTGATTTCGCTGTCTGttt  <  1:44866/61‑1 (MQ=255)
           aGTGAAATGTCATTTTGATTATTCGTTGCGCTTTTGATTTCGCTGTCTGttt  <  1:58802/52‑1 (MQ=255)
                             |                                 
CAATCAGCAACAGTGAAATGGCATTTTGACTATTCGTGGCGCTTTTGATTTCGCTGTCTGTTT  >  W3110S.gb/2267079‑2267141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: