Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,271,631	A→G	T157A (ACG→GCG)	yeiR →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,271,631	0	A	G	100.0%	112.8 / NA	62	T157A (ACG→GCG)	yeiR	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (62/0);  total (62/0)

GCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACA  >  W3110S.gb/2271598‑2271659                                  |                             gCATACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:81853/1‑60 (MQ=255) gCAGCCATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:30640/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGccc                      >  1:69675/1‑42 (MQ=37) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:59798/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:67693/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:66052/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:64091/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:61750/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:6143/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:60506/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:11023/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:58015/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:57170/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:56648/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:55416/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:54285/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:50561/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:67849/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:70689/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:72018/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:73767/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:76065/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:78742/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:80099/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:81146/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:84074/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:85857/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:88041/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:9473/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:9945/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:48651/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:11651/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:11743/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:12529/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:1269/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:16753/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:20350/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:27658/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:29335/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:31627/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:33917/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:34606/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:35933/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:48805/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:47814/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:4609/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:4605/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:4481/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:43093/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:43007/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:41510/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:36626/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:3468/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTag   >  1:20960/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTag   >  1:33140/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTag   >  1:81305/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTag   >  1:86562/1‑60 (MQ=255) gCAGACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTAAGCAAGCGCTaga  >  1:11361/1‑60 (MQ=255) gCAGACATTATTGTCGACAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:11138/1‑60 (MQ=255) gCAGACATTATTGTCGACAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:11055/1‑60 (MQ=255) gCAGACATTATTGTCCCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:18241/1‑60 (MQ=255) gCACACATTATTGTCGCCAATAAATCCGACCGTGCGACGCCCGAAAGTGAGCAAGCGCTaga  >  1:53901/1‑60 (MQ=255)                                  |                             GCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACA  >  W3110S.gb/2271598‑2271659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑