Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,293,921	C→T	P30S (CCT→TCT)	narP →	DNA‑binding response regulator in two‑component regulatory system with NarQ or NarX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,293,921	0	C	T	100.0%	55.3 / NA	32	P30S (CCT→TCT)	narP	DNA‑binding response regulator in two‑component regulatory system with NarQ or NarX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/0);  total (32/0)

TGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTG  >  W3110S.gb/2293896‑2293930                          |          tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:4839/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:87020/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:85914/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:85621/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:83660/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:81971/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:81498/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:79920/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:74580/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:72643/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:68903/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:6202/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:60052/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:54419/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:54312/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:49605/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:11939/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:45959/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:43275/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:42881/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:41074/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:38407/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:37201/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:35865/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:35138/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:33228/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:25470/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:2421/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:23704/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:22635/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:13146/1‑35 (MQ=37) tgtTCGTCAGTTACTGGAGCTTGATTCTGGCTTTg  >  1:12416/1‑35 (MQ=37)                          |          TGTTCGTCAGTTACTGGAGCTTGATCCTGGCTCTG  >  W3110S.gb/2293896‑2293930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑