Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,333,169	G→A	L433L (CTC→CTT)	yfaQ ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,333,169	0	G	A	100.0%	42.5 / NA	26	L433L (CTC→CTT)	yfaQ	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/0);  total (26/0)

GGGATAAGCAAACGCGAGTATCTGATCGTAACGCTCTCCCTGCCCCGCCTGGGCCGTGGCTT  >  W3110S.gb/2333154‑2333215                |                                               gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcgt  >  1:55227/1‑60 (MQ=255) gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcgt  >  1:85200/1‑60 (MQ=255) gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcgt  >  1:8337/1‑60 (MQ=255) gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcgt  >  1:8181/1‑60 (MQ=255) gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcgt  >  1:74897/1‑60 (MQ=255) gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcgt  >  1:73571/1‑60 (MQ=255) gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcgt  >  1:70324/1‑60 (MQ=255) gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcgt  >  1:68557/1‑60 (MQ=255) gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcgt  >  1:65814/1‑60 (MQ=255) gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcgt  >  1:65401/1‑60 (MQ=255) gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcgt  >  1:58106/1‑60 (MQ=255) gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcgt  >  1:56059/1‑60 (MQ=255) gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcgt  >  1:54195/1‑60 (MQ=255) gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcgt  >  1:43118/1‑60 (MQ=255) gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcgt  >  1:42761/1‑60 (MQ=255) gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcgt  >  1:34976/1‑60 (MQ=255) gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcgt  >  1:32313/1‑60 (MQ=255) gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcgt  >  1:31594/1‑60 (MQ=255) gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcgt  >  1:21609/1‑60 (MQ=255) gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcgt  >  1:20498/1‑60 (MQ=255) gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcgt  >  1:15561/1‑60 (MQ=255) gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcgt  >  1:1540/1‑60 (MQ=255) gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcg   >  1:12720/1‑60 (MQ=255) gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcg   >  1:32212/1‑60 (MQ=255) gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcg   >  1:2769/1‑60 (MQ=255) gggATAAGCAAACGCAAGTATCTGATCATAACGCTCTCCCTGCCCCGCCTGGGCAGTAGcg   >  1:75919/1‑60 (MQ=255)                |                                               GGGATAAGCAAACGCGAGTATCTGATCGTAACGCTCTCCCTGCCCCGCCTGGGCCGTGGCTT  >  W3110S.gb/2333154‑2333215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑