Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,337,780	A→T	pseudogene (1293/4605 nt) pseudogene (1293/4104 nt)	yfaS ← yfaS ←	ECK2220:JW2222+JW2221:b4500; hypothetical protein ECK2220:JW2222:b2228; hypothetical protein, N‑ter fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,337,780	0	A	T	100.0%	61.4 / NA	35	pseudogene (1293/4605 nt) pseudogene (1293/4104 nt)	yfaS yfaS	ECK2220:JW2222+JW2221:b4500; hypothetical protein ECK2220:JW2222:b2228; hypothetical protein, N‑ter fragment
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (35/0);  total (35/0)

CTGCTGCTATAGACCTGATTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCTACGGCATT  >  W3110S.gb/2337763‑2337824                  |                                             ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACg                             >  1:24450/1‑35 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCt                >  1:23193/1‑48 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCa    >  1:40411/1‑60 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCa    >  1:39381/1‑60 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCa    >  1:68509/1‑60 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAtt  >  1:60174/1‑62 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAtt  >  1:41540/1‑62 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAtt  >  1:4597/1‑62 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAtt  >  1:47945/1‑62 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAtt  >  1:48097/1‑62 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAtt  >  1:57483/1‑62 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAtt  >  1:871/1‑62 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAtt  >  1:65301/1‑62 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAtt  >  1:66202/1‑62 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAtt  >  1:77010/1‑62 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAtt  >  1:78804/1‑62 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAtt  >  1:83820/1‑62 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAtt  >  1:85991/1‑62 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAtt  >  1:10927/1‑62 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAtt  >  1:37989/1‑62 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAtt  >  1:37027/1‑62 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAtt  >  1:33805/1‑62 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAtt  >  1:32394/1‑62 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAtt  >  1:18846/1‑62 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAtt  >  1:18442/1‑62 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAtt  >  1:17992/1‑62 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAtt  >  1:14564/1‑62 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAtt  >  1:13879/1‑62 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAtt  >  1:13858/1‑62 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAtt  >  1:13669/1‑62 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAt   >  1:59833/1‑61 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAt   >  1:1761/1‑61 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAt   >  1:15922/1‑61 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCGACGGCAt   >  1:77180/1‑61 (MQ=255) ctgctgCTATAGACCTGTTTGCGGTAAGCAAGACGTAACTCATAACATCCGGCGACGGCAtt  >  1:39021/1‑62 (MQ=255)                  |                                             CTGCTGCTATAGACCTGATTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCTACGGCATT  >  W3110S.gb/2337763‑2337824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑