Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,342,276	A→G	Y605Y (TAT→TAC)	gyrA ←	DNA gyrase (type II topoisomerase), subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,342,276	0	A	G	100.0%	91.3 / NA	50	Y605Y (TAT→TAC)	gyrA	DNA gyrase (type II topoisomerase), subunit A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (50/0);  total (50/0)

CGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGC  >  W3110S.gb/2342237‑2342298                                        |                       cGGCCGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:64177/1‑62 (MQ=255) cGGACGCCCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:76881/1‑62 (MQ=255) cGGACGACCGCGCGCGCCCCGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:43200/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCTAATAGAcgc  >  1:18556/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:74609/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:58927/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:61259/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:68411/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:71004/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:71091/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:72605/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:73454/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:73648/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:74541/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:57317/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:78223/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:78422/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:82179/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:83392/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:83861/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:83998/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:85084/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:85668/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:88627/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:30323/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:14362/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:12390/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:1946/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:22266/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:27072/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:27913/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:28385/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:29780/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:30007/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:11863/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:36457/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:40636/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:40782/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:11722/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:4757/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:5260/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:54459/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:5678/1‑62 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcg   >  1:11720/1‑61 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcg   >  1:83723/1‑61 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcg   >  1:18484/1‑61 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcg   >  1:73369/1‑61 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcg   >  1:10183/1‑61 (MQ=255) cGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACATTCATCGAATAGAcgc  >  1:41771/1‑62 (MQ=255) cGGAAGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGGTAGACCTTCATCGAATAGAcgc  >  1:85596/1‑62 (MQ=255)                                        |                       CGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGC  >  W3110S.gb/2342237‑2342298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑