Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	254,559	A→T	A386A (GCT→GCA)	pepD ←	aminoacyl‑histidine dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	254,559	0	A	T	100.0%	78.7 / NA	44	A386A (GCT→GCA)	pepD	aminoacyl‑histidine dipeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (44/0);  total (44/0)

GCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTT  >  W3110S.gb/254542‑254603                  |                                             gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:63677/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:43539/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:45263/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:48484/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:52843/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:57895/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:5796/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:59541/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:59913/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:61007/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:61875/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:45136/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:64195/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:66610/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:71477/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:79480/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:82554/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:85798/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:86290/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:9539/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:9697/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:24318/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:11075/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:11770/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:11796/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:1301/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:15627/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:17550/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:18995/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:2262/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:2278/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:42437/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:24326/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:25302/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:28286/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:28333/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:2956/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:34757/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:35195/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt  >  1:38783/1‑62 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCtt   >  1:82786/1‑61 (MQ=37) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGCCttt  >  1:10574/1‑62 (MQ=25) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATCCTcac              >  1:80596/1‑50 (MQ=25) gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGGGAATCCAGCATGCTCACTACGTAGTCttt  >  1:24871/1‑62 (MQ=37)                  |                                             GCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTT  >  W3110S.gb/254542‑254603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑