| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 254,574 | C→T | S381S (TCG→TCA) | pepD ← | aminoacyl‑histidine dipeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 254,574 | 0 | C | T | 97.7% | 77.7 / ‑5.8 | 44 | S381S (TCG→TCA) | pepD | aminoacyl‑histidine dipeptidase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (43/0); minor base G (1/0); total (44/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTT > W3110S.gb/254542‑254603 | gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:63677/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:43539/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:45263/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:48484/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:52843/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:57895/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:5796/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:59541/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:59913/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:61007/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:61875/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:45136/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:64195/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:66610/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:71477/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:79480/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:82554/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:85798/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:86290/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:9539/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:9697/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:24318/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:11075/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:11770/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:11796/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:1301/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:15627/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:17550/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:18995/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:2262/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:2278/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:42437/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:24326/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:25302/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:28286/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:28333/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:2956/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:34757/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:35195/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCttt > 1:38783/1‑62 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGTCtt > 1:82786/1‑61 (MQ=37)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATGCTCACTACGTAGCCttt > 1:10574/1‑62 (MQ=25)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGTGAATCCAGCATCCTcac > 1:80596/1‑50 (MQ=25)gCTTCTGTTTTCGCACCTGCCAGTTTACCCAGGGAATCCAGCATGCTCACTACGTAGTCttt > 1:24871/1‑62 (MQ=37) | GCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTT > W3110S.gb/254542‑254603 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |