Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,401,816 C→T A99A (GCG→GCA)  nuoH ← NADH:ubiquinone oxidoreductase, membrane subunit H

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,401,8160CT100.0% 145.1 / NA 77A99A (GCG→GCA) nuoHNADH:ubiquinone oxidoreductase, membrane subunit H
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (77/0);  total (77/0)

GATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCA  >  W3110S.gb/2401771‑2401832
                                             |                
gATGTTCAGGTCGGCACCCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:25127/1‑62 (MQ=255)
gATGTTCAGGTCGGCACCCACCCAACCCGGACTGACTGGCACAATTG‑AAAGGCcagcagca  >  1:72497/1‑61 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:5789/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:67541/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:67501/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:66501/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:65658/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:64568/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:62716/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:62316/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:60401/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:67702/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:55763/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:55717/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:53978/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:53432/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:5237/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:51367/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:50712/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:11995/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:80908/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:9302/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:914/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:85492/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:84668/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:8380/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:82989/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:8264/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:81019/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:67701/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:79390/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:73447/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:73096/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:72461/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:71256/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:68664/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:50237/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:27606/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:33809/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:33755/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:50005/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:32614/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:31578/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:30270/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:29443/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:28463/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:32628/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:2693/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:2483/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:24314/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:2145/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:20156/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:20024/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:14866/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:14479/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:36594/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:48783/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:48482/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:47958/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:46474/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:43694/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:43570/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:43488/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:42685/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:41365/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:39257/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:37425/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:36948/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:36810/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:34237/1‑62 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagc   >  1:42055/1‑61 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagc   >  1:25925/1‑61 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagc   >  1:84845/1‑61 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagc   >  1:19987/1‑61 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcag    >  1:60625/1‑60 (MQ=255)
gATGTTCAGGTCGGCAACCACCCAACACGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:8937/1‑62 (MQ=255)
gATGTTCAGGTCGCCAACCACCCAACCCGGACTGACTGGCACAATTGCAAAGGCcagcagca  >  1:79251/1‑62 (MQ=255)
                                             |                
GATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCA  >  W3110S.gb/2401771‑2401832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑