Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,433,118 T→C S40S (TCA→TCG)  argT ← lysine/arginine/ornithine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,433,1180TC100.0% 115.5 / NA 60S40S (TCA→TCG) argTlysine/arginine/ornithine transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (60/0);  total (60/0)

ACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACG  >  W3110S.gb/2433092‑2433153
                          |                                   
agaaaaTCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:58008/3‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGgcgcat                        >  1:4363/1‑38 (MQ=25)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGCTa    >  1:16991/1‑60 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATAc   >  1:12009/1‑61 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATAc   >  1:85056/1‑61 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATAc   >  1:40294/1‑61 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATAc   >  1:37038/1‑61 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATAc   >  1:35621/1‑61 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATAc   >  1:20752/1‑61 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATAc   >  1:18786/1‑61 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATAc   >  1:1072/1‑61 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATAc   >  1:15829/1‑61 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:69216/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:55510/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:55742/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:57073/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:14445/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:59188/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:59498/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:62864/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:64615/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:65928/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:6783/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:68464/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:54065/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:70306/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:74838/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:75451/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:77488/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:78269/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:78681/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:80543/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:84656/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:85330/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:9205/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:9487/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:35944/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:168/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:23079/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:26701/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:2704/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:28114/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:30102/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:30727/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:31949/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:3259/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:16423/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:55123/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:36009/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:38137/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:38550/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:39013/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:15759/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:40374/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:41316/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:15554/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:44966/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:46035/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACGAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:50361/1‑62 (MQ=255)
acaaAATCACCTTTAGCATCTTTCGACAAGAACGGCGCATAGGTGGTATCGGTTCCGATACg  >  1:22695/1‑62 (MQ=255)
                          |                                   
ACAAAATCACCTTTAGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACG  >  W3110S.gb/2433092‑2433153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑