Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | W3110S.gb | 2,436,521 | A→G | L204L (TTG→CTC) | dedD ← | conserved hypothetical protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 2,436,521 | 0 | A | G | 98.8% | 164.3 / NA | 86 | L204L (TTG→CTC) | dedD | conserved hypothetical protein |
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (0/85); total (0/86) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 |
TTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCG > W3110S.gb/2436472‑2436532 | ttCGGTGTATAGCCCATTACCACTCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:15687/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACTCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:15804/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTTAGCTCACCCAGCg < 1:769/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:59866/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:69964/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:69481/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:69155/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:67753/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:67448/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:66396/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:65428/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:62514/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:6226/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:72316/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:59814/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:56005/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:52040/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:51398/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:50871/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:50568/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:50198/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:49958/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:76237/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:87409/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:83891/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:81560/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:79203/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:78771/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:78712/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:78642/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:77586/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:76922/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:71961/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:76036/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:75078/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:74729/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:74722/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:73101/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:73083/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:13017/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:72120/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:2464/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:35390/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:32198/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:31677/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:3088/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:30456/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:30125/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:29038/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:24971/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:49158/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:19895/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:18648/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:1517/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:145/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:1444/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:13720/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:1013/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:36111/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:49576/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:49055/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:45999/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:45477/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:44661/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:44632/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:41798/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:41221/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:35878/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:39273/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:37172/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAACTCACCCAGCg < 1:24337/61‑1 (MQ=255) ttCGGTGTATAGCCCATTACCACGCCACTTAAGACAGAAAGCTGCTTGAGCTCACCCAGCg < 1:25646/61‑1 (MQ=255) tCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:84179/60‑1 (MQ=255) tCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:83948/60‑1 (MQ=255) tCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:80585/60‑1 (MQ=255) tCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:80170/60‑1 (MQ=255) tCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:28644/60‑1 (MQ=255) tCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:51985/60‑1 (MQ=255) tCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:38994/60‑1 (MQ=255) tCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:7680/60‑1 (MQ=255) tCGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:4158/60‑1 (MQ=255) cGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAggg < 1:31270/59‑3 (MQ=255) cGGTGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:17025/59‑1 (MQ=255) aCGCCACTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:14895/40‑1 (MQ=255) aCTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:36200/35‑1 (MQ=25) aCTTAAGCCAGAAAGCTGCTTGAGCTCACCCAGCg < 1:87718/35‑1 (MQ=25) | TTCGGCGTATAGCCCATTACCACGCCACTTAAGCCAGAAAGTTGCTTCAACTCACCCAGCG > W3110S.gb/2436472‑2436532 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |