Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,454,045	C→A	intergenic (‑159/‑7)	prmB ← / → yfcN	N5‑glutamine methyltransferase/conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,454,045	0	C	A	100.0%	97.7 / NA	53	intergenic (‑159/‑7)	prmB/yfcN	N5‑glutamine methyltransferase/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (0/53);  total (0/53)

AAATTAGAAATAGAGACAGATAAATGAAAAAGAAAACAACACTT  >  W3110S.gb/2454029‑2454072                 |                            aaaTTAGAAATAGAGAAAGATAAATGAAAAATAAAACAACACtt  <  1:49959/44‑1 (MQ=38) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:65699/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:48951/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:82946/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:53746/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:81401/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:54712/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:56523/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:60917/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:63057/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:81306/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:80405/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:48928/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:67473/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:6819/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:68348/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:71319/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:73543/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:73744/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:76639/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:77296/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:77630/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:77790/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:33390/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:9792/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:15719/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:1612/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:21462/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:25594/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:26161/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:27960/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:87984/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:29632/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:32495/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:33311/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:84981/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:39808/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:39977/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:42048/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:42133/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:43723/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:44053/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:44151/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:47834/44‑1 (MQ=255) aaaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:48620/44‑1 (MQ=255)  aaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:52493/43‑1 (MQ=255)  aaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:65033/43‑1 (MQ=255)  aaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:53747/43‑1 (MQ=255)  aaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:38165/43‑1 (MQ=255)  aaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:29117/43‑1 (MQ=255)  aaTTAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:14395/43‑1 (MQ=255)    ttAGAAATAGAGAAAGATAAATGAAAAAGAAAACAACACtt  <  1:12231/41‑1 (MQ=255)     tAGAAATAGAGAAATATAAATGAAAAAGAAAACAACACtt  <  1:64881/40‑1 (MQ=38)                 |                            AAATTAGAAATAGAGACAGATAAATGAAAAAGAAAACAACACTT  >  W3110S.gb/2454029‑2454072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑