Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,463,277	G→A	I443I (ATC→ATT)	yfcX ←	fused enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, dodecenoyl‑CoA D‑isomerase, and 3‑hydroxyacyl‑CoA dehydrogenase, subunit of anaerobic fatty acid oxidation complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,463,277	0	G	A	100.0%	42.0 / NA	25	I443I (ATC→ATT)	yfcX	fused enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, dodecenoyl‑CoA D‑isomerase, and 3‑hydroxyacyl‑CoA dehydrogenase, subunit of anaerobic fatty acid oxidation complex
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/25);  total (0/25)

TTTTTCCACCGGACTGAAGAAATGCAGGCCGATAACTTGCTCAGGTCGCGTGGCGTGAGCGG  >  W3110S.gb/2463247‑2463308                               |                                tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg  <  1:55198/62‑1 (MQ=255) tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg  <  1:9186/62‑1 (MQ=255) tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg  <  1:87463/62‑1 (MQ=255) tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg  <  1:84461/62‑1 (MQ=255) tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg  <  1:77877/62‑1 (MQ=255) tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg  <  1:75952/62‑1 (MQ=255) tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg  <  1:71261/62‑1 (MQ=255) tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg  <  1:69897/62‑1 (MQ=255) tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg  <  1:66612/62‑1 (MQ=255) tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg  <  1:55590/62‑1 (MQ=255) tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg  <  1:55344/62‑1 (MQ=255) tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg  <  1:15486/62‑1 (MQ=255) tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg  <  1:53387/62‑1 (MQ=255) tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg  <  1:4688/62‑1 (MQ=255) tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg  <  1:4494/62‑1 (MQ=255) tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg  <  1:30970/62‑1 (MQ=255) tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg  <  1:27122/62‑1 (MQ=255) tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg  <  1:26837/62‑1 (MQ=255) tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg  <  1:25230/62‑1 (MQ=255) tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg  <  1:16149/62‑1 (MQ=255) tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg  <  1:16035/62‑1 (MQ=255) tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGGGAgcgg  <  1:1657/62‑1 (MQ=255) tttttCCACCGGACTGAAGAAATGCAGGCAAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg  <  1:68679/62‑1 (MQ=255)  ttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg  <  1:20482/61‑1 (MQ=255)   tttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg  <  1:59630/60‑1 (MQ=255)                               |                                TTTTTCCACCGGACTGAAGAAATGCAGGCCGATAACTTGCTCAGGTCGCGTGGCGTGAGCGG  >  W3110S.gb/2463247‑2463308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑