Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,467,870	T→C	D375D (GAT→GAC)	fadL →	long‑chain fatty acid outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,467,870	0	T	C	100.0%	13.8 / NA	10	D375D (GAT→GAC)	fadL	long‑chain fatty acid outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/0);  total (10/0)

CGTACCGGTATCGCCTTTGATGACAGCCCAGTTCCTGCACAGAATCGTTCTATCTCCATTC  >  W3110S.gb/2467850‑2467910                     |                                         cGTACCGGTATCGCTTATGACGACAGCCCGGTTCCGGCAGATAAACGTTCTATTTCCATTc  >  1:14043/1‑61 (MQ=255) cGTACCGGTATCGCTTATGACGACAGCCCGGTTCCGGCAGATAAACGTTCTATTTCCATTc  >  1:27162/1‑61 (MQ=255) cGTACCGGTATCGCTTATGACGACAGCCCGGTTCCGGCAGATAAACGTTCTATTTCCATTc  >  1:27776/1‑61 (MQ=255) cGTACCGGTATCGCTTATGACGACAGCCCGGTTCCGGCAGATAAACGTTCTATTTCCATTc  >  1:50768/1‑61 (MQ=255) cGTACCGGTATCGCTTATGACGACAGCCCGGTTCCGGCAGATAAACGTTCTATTTCCATTc  >  1:57716/1‑61 (MQ=255) cGTACCGGTATCGCTTATGACGACAGCCCGGTTCCGGCAGATAAACGTTCTATTTCCATTc  >  1:58003/1‑61 (MQ=255) cGTACCGGTATCGCTTATGACGACAGCCCGGTTCCGGCAGATAAACGTTCTATTTCCATTc  >  1:607/1‑61 (MQ=255) cGTACCGGTATCGCTTATGACGACAGCCCGGTTCCGGCAGATAAACGTTCTATTTCCATTc  >  1:62054/1‑61 (MQ=255) cGTACCGGTATCGCTTATGACGACAGCCCGGTTCCGGCAGATAAACGTTCTATTTCCATTc  >  1:77330/1‑61 (MQ=255) cGTACCGGTATCGCTTATGACGACAGCCCGGTTCCGGCAGATAAACGTTCTATTTCCATTc  >  1:77687/1‑61 (MQ=255)                     |                                         CGTACCGGTATCGCCTTTGATGACAGCCCAGTTCCTGCACAGAATCGTTCTATCTCCATTC  >  W3110S.gb/2467850‑2467910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑