Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,469,710	A→G	I248I (ATT→ATC)	vacJ ←	predicted lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,469,710	0	A	G	100.0%	116.4 / NA	61	I248I (ATT→ATC)	vacJ	predicted lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/61);  total (0/61)

TTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCC  >  W3110S.gb/2469695‑2469755                |                                              ttCTTATTCAGAATCGATATCTTTTCAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:5723/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTTAATCGCTTGCGCGTTCGGATTTTcc  <  1:66996/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:76045/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:57845/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:58820/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:6079/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:64272/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:64362/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:65268/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:66088/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:67821/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:70142/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:70753/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:71700/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:73864/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:75085/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:5473/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:77498/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:78705/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:80462/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:80532/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:81285/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:81551/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:83218/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:83838/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:84209/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:85121/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:85821/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:86856/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:86986/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:88642/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:50235/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:11232/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:13998/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:16250/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:17928/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:20276/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:2069/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:21510/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:21621/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:21756/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:22738/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:27216/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:28901/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:32620/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:32917/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:33438/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:34051/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:34344/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:34813/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:35145/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:36903/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:40877/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:41035/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:42621/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:4621/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:48011/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:48692/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:4925/61‑1 (MQ=255) ttCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:10643/61‑1 (MQ=255)  tCTTATTCAGAATCGATATCTTTTAAATCATCCTGAATCGCTTGCGCGTTCGGATTTTcc  <  1:33401/60‑1 (MQ=255)                |                                              TTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCC  >  W3110S.gb/2469695‑2469755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑