Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,490,667	T→C	F283S (TTT→TCT)	evgS →	hybrid sensory histidine kinase in two‑component regulatory system with EvgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,490,667	0	T	C	100.0%	63.8 / NA	36	F283S (TTT→TCT)	evgS	hybrid sensory histidine kinase in two‑component regulatory system with EvgA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/36);  total (0/36)

AAATGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGA  >  W3110S.gb/2490611‑2490672                                                         |      aaaTGAAGTTCGCTATGAAGTATCACAAAATTTGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:46501/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:60221/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:10101/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:41928/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:4434/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:46120/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:47952/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:51932/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:52347/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:40303/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:64789/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:65553/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:65622/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:72763/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:72968/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:75303/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:83014/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:86701/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:32187/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:13532/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:13671/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:15808/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:19382/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:19832/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:20154/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:2142/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:23981/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:27358/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:28483/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:28586/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:3076/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:30782/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:31089/62‑1 (MQ=255) aaaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:38768/62‑1 (MQ=255)  aaTGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTCTCTGa  <  1:2240/61‑1 (MQ=255)                            aaaTTGGCTTGATACAGTAAACCTGGCCTCTCTGa  <  1:63032/35‑1 (MQ=37)                                                         |      AAATGAAGTTCGCTATGAAGTATCACAAAATTGGCTTGATACAGGAAACCTGGCCTTTCTGA  >  W3110S.gb/2490611‑2490672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑