Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,503,253	T→G	R163R (CGA→CGC)	yfdZ ←	prediected aminotransferase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,503,253	0	T	G	100.0%	136.4 / NA	73	R163R (CGA→CGC)	yfdZ	prediected aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (73/0);  total (73/0)

TCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGC  >  W3110S.gb/2503231‑2503292                       |                                        tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCtt          >  1:34360/1‑54 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTccaccacc  >  1:43042/1‑60 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTccacca    >  1:27536/1‑60 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTc         >  1:78042/1‑55 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAg   >  1:35640/1‑61 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAg   >  1:10955/1‑61 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAg   >  1:73965/1‑61 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAg   >  1:49507/1‑61 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAg   >  1:62822/1‑61 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAg   >  1:66554/1‑61 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:71264/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:56666/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:70037/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:6820/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:67957/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:67710/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:63499/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:6311/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:60363/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:5787/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:57024/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:5201/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:77682/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:9098/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:86877/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:86841/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:85015/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:79023/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:77967/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:77940/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:77874/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:7135/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:76755/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:76008/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:74711/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:74666/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:74215/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:74170/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:72333/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:25555/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:32457/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:32449/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:29572/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:27981/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:26941/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:26573/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:26513/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:25791/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:53168/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:25205/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:23546/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:21196/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:20903/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:189/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:17516/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:13179/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:43657/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:53637/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:52126/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:51745/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:50968/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:48789/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:48636/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:47125/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:45535/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:42550/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:41270/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:39319/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:39239/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:3859/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:38245/1‑62 (MQ=255) tCGGATAACTTTCACGAATGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTAccaccacc  >  1:73813/1‑60 (MQ=255) tCGGATAACTTTCACGAAAGGCGCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGc  >  1:35440/1‑62 (MQ=255)                       |                                        TCGGATAACTTTCACGAATGGCTCGCTCCAGTTCGTTGAAGAAATCGACACCTTCCACCAGC  >  W3110S.gb/2503231‑2503292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑