Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,534,240	C→T	Q461Q (CAG→CAA)	ligA ←	DNA ligase, NAD(+)‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,534,240	0	C	T	100.0%	146.8 / NA	79	Q461Q (CAG→CAA)	ligA	DNA ligase, NAD(+)‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (79/0);  total (79/0)

CTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCC  >  W3110S.gb/2534206‑2534266                                   |                           ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGc         >  1:23310/3‑54 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:58977/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:68738/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:68376/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:65002/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:64621/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:63812/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:63762/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:60589/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:59400/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:69503/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:58632/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:57678/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:57072/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:56724/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:5541/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:5471/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:53865/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:53608/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:10367/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:77738/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:9939/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:9879/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:88530/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:85364/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:82595/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:81142/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:79780/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:78595/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:77827/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:68741/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:76350/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:75062/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:74940/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:74883/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:74694/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:73659/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:71662/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:53303/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:19892/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:51082/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:29017/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:26805/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:26587/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:24974/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:24605/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:22850/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:22499/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:21444/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:30176/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:19151/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:18559/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:17885/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:16089/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:14888/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:13751/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:12124/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:11081/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:10725/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:30928/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:50117/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:50062/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:48522/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:47936/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:46482/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:39260/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:37898/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:37234/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:36474/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:35787/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:33080/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:32843/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:32363/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:32323/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:31829/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:3069/3‑61 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTc   >  1:58395/3‑60 (MQ=255) ccgCCGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTc   >  1:54476/3‑60 (MQ=255) ccaccGGAGTGTGGACATATTCTTTTTCAACCAGTTGATCGATGATTTTGTCGCCCATTcc  >  1:9253/4‑61 (MQ=255)                                   |                           CTGCCGGAGTATGGACATATTCTTTTTCAACCAGCTGATCGATGATTTTGTCGCCCATTCC  >  W3110S.gb/2534206‑2534266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑