Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,551,680	A→G	A154A (GCA→GCG)	yfeU →	predicted PTS component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,551,680	0	A	G	100.0%	66.2 / NA	37	A154A (GCA→GCG)	yfeU	predicted PTS component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/0);  total (37/0)

GCCGTATGTGATTGCCGGACTGGAATACGCACGCCAGCTCGGCTGCCGCACAGTGGGAATTTC  >  W3110S.gb/2551650‑2551712                               |                                 gCCGTATGTGATTGCTGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:80308/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCTGCTGCCGTACAGTGGGCAttt   >  1:36977/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGc                      >  1:10410/1‑43 (MQ=25) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTTCCGTACAGTGGGCAtt    >  1:49124/1‑61 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGcc                  >  1:34780/1‑47 (MQ=37) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:67579/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:49849/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:52016/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:57031/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:57660/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:66130/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:37949/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:68397/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:71377/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:7333/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:7665/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:84360/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:84809/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:955/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:23468/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:10467/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:13950/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:15006/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:15398/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:17225/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:19243/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:46823/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:2426/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:28259/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:28843/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:28923/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:31005/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:32841/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:33346/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAttt   >  1:37380/1‑62 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCGCCAACTCGGCTGCCGTACAGTGGGCAtt    >  1:75101/1‑61 (MQ=255) gCCGTATGTGATTGCCGGGCTGGAATACGCGCG‑CAACTCGGCTGCCGTACAGTGGGCATTTc  >  1:78487/1‑62 (MQ=255)                               |                                 GCCGTATGTGATTGCCGGACTGGAATACGCACGCCAGCTCGGCTGCCGCACAGTGGGAATTTC  >  W3110S.gb/2551650‑2551712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑