Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,557,449	A→G	L46L (TTG→CTG)	ypeA ←	predicted acyltransferase with acyl‑CoA N‑acyltransferase domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,557,449	0	A	G	100.0%	78.5 / NA	42	L46L (TTG→CTG)	ypeA	predicted acyltransferase with acyl‑CoA N‑acyltransferase domain
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/42);  total (0/42)

CCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCAC  >  W3110S.gb/2557446‑2557507    |                                                           ccAGAAACAAACTGACGTTATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:25132/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACTCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:64235/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:73528/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:46355/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:50161/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:52211/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:52933/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:59985/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:60153/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:64080/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:69383/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:71217/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:4421/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:75438/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:77301/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:78606/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:80699/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:83327/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:8448/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:85217/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:87987/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:88567/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:30392/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:16324/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:17118/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:17980/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:20996/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:21719/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:23778/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:2582/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:28703/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:1219/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:31451/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:32973/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:35570/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:36934/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:38014/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:38305/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:38349/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:38627/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATGTCCATTTCCGGATCGTTCCAc  <  1:42517/62‑1 (MQ=255) ccAGAAACAAACTGACGTCATGGTTCATCTTACGCTCAATCTCCATTTCCGGATCGTTCCAc  <  1:29528/62‑1 (MQ=255)    |                                                           CCAAAAACAAACTGACGTCATGGTTCATCTTACGCTCGATGTCCATTTCCGGATCGTTCCAC  >  W3110S.gb/2557446‑2557507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑