Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	256,895	T→A	R123R (CGT→CGA)	frsA →	hydrolase, binds to enzyme IIA(Glc)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	256,895	0	T	A	100.0%	68.1 / NA	38	R123R (CGT→CGA)	frsA	hydrolase, binds to enzyme IIA(Glc)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (0/38);  total (0/38)

AACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGC  >  W3110S.gb/256852‑256912                                            |                  aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:73407/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:10542/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:51738/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:53084/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:5579/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:56065/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:56130/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:58162/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:66925/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:70095/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:51579/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:74702/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:77018/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:77022/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:79565/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:84218/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:84949/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:88643/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:9496/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:3674/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:14572/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:16729/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:19227/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:21087/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:21203/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:23020/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:31950/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:49148/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:37929/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:39030/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:39339/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:40774/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:42187/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:43305/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:44857/61‑1 (MQ=255) aacaaAAAGCCTGTGCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:46516/61‑1 (MQ=255)            tgtgCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:20808/50‑1 (MQ=255)             gtgCGGAAGAAGATCCGCAACTCAGCGGTCGACACTGGCTGCATGCGGc  <  1:76907/49‑1 (MQ=255)                                            |                  AACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGC  >  W3110S.gb/256852‑256912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑