Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,592,650	A→G	D622D (GAT→GAC)	ypfI ←	predicted hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,592,650	0	A	G	100.0%	34.0 / NA	20	D622D (GAT→GAC)	ypfI	predicted hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/20);  total (0/20)

CATAACTGCGCATCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTC  >  W3110S.gb/2592639‑2592700            |                                                   cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc  <  1:45753/62‑1 (MQ=255) cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc  <  1:86120/62‑1 (MQ=255) cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc  <  1:73388/62‑1 (MQ=255) cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc  <  1:73248/62‑1 (MQ=255) cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc  <  1:6384/62‑1 (MQ=255) cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc  <  1:58815/62‑1 (MQ=255) cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc  <  1:58778/62‑1 (MQ=255) cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc  <  1:5554/62‑1 (MQ=255) cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc  <  1:51921/62‑1 (MQ=255) cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc  <  1:10749/62‑1 (MQ=255) cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc  <  1:44449/62‑1 (MQ=255) cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc  <  1:35170/62‑1 (MQ=255) cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc  <  1:33085/62‑1 (MQ=255) cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc  <  1:2865/62‑1 (MQ=255) cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc  <  1:24700/62‑1 (MQ=255) cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc  <  1:15803/62‑1 (MQ=255) cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc  <  1:14591/62‑1 (MQ=255) cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc  <  1:1169/62‑1 (MQ=255) cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc  <  1:11579/62‑1 (MQ=255)  aTAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc  <  1:60878/61‑1 (MQ=255)            |                                                   CATAACTGCGCATCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTC  >  W3110S.gb/2592639‑2592700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑