Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,598,970	C→A	R137R (CGG→AGG)	gcvR →	DNA‑binding transcriptional repressor, regulatory protein accessory to GcvA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,598,970	0	C	A	100.0%	106.2 / NA	60	R137R (CGG→AGG)	gcvR	DNA‑binding transcriptional repressor, regulatory protein accessory to GcvA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (60/0);  total (60/0)

ATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGC  >  W3110S.gb/2598965‑2599025      |                                                        aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:68323/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:53121/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:53713/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:54439/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:56726/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:56780/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:58563/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:61590/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:62901/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:63139/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:63262/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:63710/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:63939/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:64867/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:6596/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:49535/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:69396/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:71652/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:71928/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:73210/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:73909/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:75320/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:77404/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:80478/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:80915/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:83760/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:83853/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:86280/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:86715/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:87398/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:33192/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:14066/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:18908/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:20365/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:21131/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:22146/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:2413/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:24429/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:24615/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:25049/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:29403/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:30258/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:30831/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:31483/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:31651/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:11824/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:34613/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:35897/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:36705/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:40200/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:41728/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:4290/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:4355/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:43860/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:44486/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:44706/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:45937/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:4603/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:48292/1‑61 (MQ=255) aTGAAAGGGCTGCGCAGTTGCATATTCAGATAACTGCCCACAGCCCCGCATCTGCGGACgc  >  1:49165/1‑61 (MQ=255)      |                                                        ATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGC  >  W3110S.gb/2598965‑2599025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑