Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,643,122	A→G	F133F (TTT→TTC)	ndk ←	multifunctional nucleoside diphosphate kinase, apyrimidinic endonuclease, and 3'‑phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,643,122	0	A	G	100.0%	103.2 / NA	54	F133F (TTT→TTC)	ndk	multifunctional nucleoside diphosphate kinase, apyrimidinic endonuclease, and 3'‑phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/54);  total (0/54)

AATTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCAAAGAAATAAGCGATTTCGCG  >  W3110S.gb/2643084‑2643142                                       |                     aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:73960/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:10901/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:62341/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:63256/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:63810/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:64523/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:65751/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:6933/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:6938/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:69965/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:70007/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:71479/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:71536/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:71786/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:57217/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:74787/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:74810/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:74929/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:76200/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:77516/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:77890/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:81908/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:83282/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:84684/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:87183/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:87699/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:88258/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:32162/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:16144/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:16392/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:18239/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:18668/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:19925/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:20007/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:20051/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:2037/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:21000/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:21016/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:29610/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:30423/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:56863/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:35801/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:37861/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:38129/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:41778/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:43431/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:48048/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:49103/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:49637/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:52949/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:54997/59‑1 (MQ=255) aaTTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:55540/59‑1 (MQ=255)  attattAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:32228/58‑1 (MQ=255)  attattAACGGGTGCGCGGGCACACTTCGCCTTCGCCGAAGAAATAAGCGATTTcgcg  <  1:82819/58‑1 (MQ=255)                                       |                     AATTATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCAAAGAAATAAGCGATTTCGCG  >  W3110S.gb/2643084‑2643142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑