Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,656,629	G→A	D321D (GAC→GAT)	hscA ←	DnaK‑like molecular chaperone specific for IscU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,656,629	0	G	A	100.0%	114.4 / NA	62	D321D (GAC→GAT)	hscA	DnaK‑like molecular chaperone specific for IscU
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/62);  total (0/62)

AGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGC  >  W3110S.gb/2656578‑2656638                                                    |          aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:57916/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:39626/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:10711/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:42502/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:44783/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:4598/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:50506/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:51014/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:51139/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:51544/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:52346/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:54067/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:54436/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:55539/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:57748/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:42293/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:61128/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:61872/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:63761/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:64189/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:66340/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:6841/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:7605/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:78479/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:78579/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:8123/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:86129/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:86251/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:9279/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:26764/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:10838/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:12256/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:12283/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:127/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:14434/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:14925/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:19269/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:21140/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:21456/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:2162/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:21652/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:25120/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:38540/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:26933/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:34713/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:35846/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:35745/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:35359/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:351/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:35029/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:34982/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:29088/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:333/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:31704/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:31559/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:30787/61‑1 (MQ=255) aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:3040/61‑1 (MQ=255)  gAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:48044/60‑1 (MQ=255)          caccaTCACCACTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:58999/52‑1 (MQ=39)                 accacTTCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:75251/45‑1 (MQ=38)                       tCCAGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:25886/39‑1 (MQ=37)                          aGCACTTCATCAGCTTCTACGCCCGCATCTTTCAgc  <  1:6479/36‑1 (MQ=25)                                                    |          AGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGC  >  W3110S.gb/2656578‑2656638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑