Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,679,425	C→A	L659L (CTG→CTT)	yphG ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,679,425	0	C	A	100.0%	78.9 / NA	45	L659L (CTG→CTT)	yphG	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (45/0);  total (45/0)

TCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACACAGACCGCGCCACTGC  >  W3110S.gb/2679379‑2679440                                               |                tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:68918/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:61619/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:61812/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:62669/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:63298/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:64455/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:64776/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:66042/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:66404/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:67836/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:67868/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:15685/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:69563/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:73150/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:76434/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:76447/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:81134/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:81479/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:82566/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:85670/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:87113/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:87896/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:457/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:25630/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:26835/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:28110/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:31044/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:20975/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:18893/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:44833/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:1535/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:47852/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:50515/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:51813/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:53606/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:54718/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:57261/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTgc  >  1:56136/1‑62 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTg   >  1:9618/1‑61 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTg   >  1:1870/1‑61 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTg   >  1:34413/1‑61 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTg   >  1:34570/1‑61 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTg   >  1:35086/1‑61 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTg   >  1:47740/1‑61 (MQ=255) tCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACAAAGACCTCGCCACTg   >  1:55353/1‑61 (MQ=255)                                               |                TCAGTTGCCCGGCGGTCATCAGGGCGTTAACGTCGCGTCCCTGACACAGACCGCGCCACTGC  >  W3110S.gb/2679379‑2679440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑