Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,693,210	C→A	A330A (GCG→GCT)	purL ←	phosphoribosylformyl‑glycineamide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,693,210	0	C	A	100.0%	126.0 / NA	70	A330A (GCG→GCT)	purL	phosphoribosylformyl‑glycineamide synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (70/0);  total (70/0)

GGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGA  >  W3110S.gb/2693192‑2693253                   |                                            ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCgg   >  1:65407/1‑61 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:58770/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:68667/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:67598/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:66993/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:66283/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:65667/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:61262/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:5938/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:69795/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:58220/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:57964/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:56180/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:53870/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:53833/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:51876/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:51835/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:76131/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:8747/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:83601/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:83174/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:83037/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:80935/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:80784/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:793/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:76711/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:10538/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:7495/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:74892/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:73702/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:72906/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:7232/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:71987/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:7177/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:16335/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:33197/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:32852/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:32594/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:25990/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:23616/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:18381/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:17890/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:17810/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:48086/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:16161/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:14971/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:11649/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:11509/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:11236/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:10786/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:10680/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:10533/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:44303/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:49630/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:47558/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:47218/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:46134/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:4533/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:45318/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:44962/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:44636/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:41291/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:41064/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:4042/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:39098/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:36911/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:35063/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:3447/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCGCCACGTCCGGTGGCACCTTAATCGCGGa  >  1:9425/1‑62 (MQ=255) ggAGAAACCAACCAGACCAGCTTTCGGCTTTGCCCCACGTCCGGTGGCACCTTCATCGCGGa  >  1:68059/1‑62 (MQ=255)                   |                                            GGAGAAACCAACCAGACCCGCTTTCGGCTTTGCGCCGCGCCCGGTGGCACCTTCATCGCGGA  >  W3110S.gb/2693192‑2693253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑