Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,709,636	T→A	R187R (CGT→CGA)	nadB →	quinolinate synthase, L‑aspartate oxidase (B protein) subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,709,636	0	T	A	100.0%	115.2 / NA	64	R187R (CGT→CGA)	nadB	quinolinate synthase, L‑aspartate oxidase (B protein) subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (64/0);  total (64/0)

GAACCGTAATAAAGAAACGGTGGAAACCTGCCACGCAAAAGCGGTGGTGC  >  W3110S.gb/2709630‑2709679       |                                            gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGc          >  1:36078/1‑42 (MQ=38) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:69277/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:10035/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:47672/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:49129/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:51369/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:53428/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:56919/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:57484/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:60627/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:60896/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:61201/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:62095/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:64817/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:67206/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:69242/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:42694/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:70436/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:71097/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:74180/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:77322/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:77900/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:81033/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:81324/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:83349/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:85431/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:85540/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:85572/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:85751/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:85761/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:87454/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:24758/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:10610/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:10730/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:10824/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:1174/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:11945/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:15234/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:15941/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:17435/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:19254/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:21855/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:22333/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:23188/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:24528/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:39813/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:32591/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:38438/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:36817/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:36125/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:3423/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:33910/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:32956/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:24984/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:32480/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:29245/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:29236/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:29147/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggtac  >  1:26626/1‑48 (MQ=255) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggta   >  1:85053/1‑48 (MQ=38) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggta   >  1:33066/1‑48 (MQ=38) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggta   >  1:75659/1‑48 (MQ=38) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggta   >  1:24067/1‑48 (MQ=38) gAACCGAAATAAAGAAACGGTTGAAACCTGCCACGCAAAAGCagtggta   >  1:67534/1‑48 (MQ=38)       |                                            GAACCGTAATAAAGAAACGGTGGAAACCTGCCACGCAAAAGCGGTGGTGC  >  W3110S.gb/2709630‑2709679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑