Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,713,629	G→A	A116A (GCC→GCT)	yfiE ←	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,713,629	0	G	A	100.0%	91.0 / NA	50	A116A (GCC→GCT)	yfiE	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/50);  total (0/50)

CTTTTGGGGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGGTAAGAGAGCAACGTT  >  W3110S.gb/2713622‑2713683        |                                                       cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:70044/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:56302/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:59049/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:11561/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:64159/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:64173/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:64493/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:65542/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:65979/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:66684/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:66930/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:70008/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:6300/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:7181/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:78421/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:79312/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:8338/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:84476/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:84999/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:87223/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:88125/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:911/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:9527/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:2771/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:11764/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:16555/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:18255/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:20155/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:20796/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:23715/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:24238/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:27170/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:27321/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:54829/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:32904/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:36042/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:37801/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:37888/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:38340/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:42746/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:51044/62‑1 (MQ=255) cTTTTGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:53453/62‑1 (MQ=255)  ttttGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:6859/61‑1 (MQ=255)  ttttGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:6831/61‑1 (MQ=255)  ttttGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:25681/61‑1 (MQ=255)  ttttGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:81739/61‑1 (MQ=255)  ttttGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:84745/61‑1 (MQ=255)  ttttGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:46505/61‑1 (MQ=255)  ttttGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:87790/61‑1 (MQ=255)  ttttGGAGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGATAAGAGAGCAACGtt  <  1:52532/61‑1 (MQ=255)        |                                                       CTTTTGGGGCGCGTTGCCGAAAACGCTGCAACACCTGCGGCATTCGGTAAGAGAGCAACGTT  >  W3110S.gb/2713622‑2713683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑