Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,716,109	G→A	intergenic (+10/+38)	ung → / ← yfiF	uracil‑DNA‑glycosylase/predicted methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,716,109	0	G	A	100.0%	50.3 / NA	30	intergenic (+10/+38)	ung/yfiF	uracil‑DNA‑glycosylase/predicted methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/0);  total (30/0)

ATGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCG  >  W3110S.gb/2716070‑2716117                                        |         aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGcc   >  1:68699/1‑47 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:50017/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:9219/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:8330/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:82431/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:73726/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:72890/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:71874/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:71563/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:70129/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:70016/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:67023/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:65819/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:57806/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:54513/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:11932/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:49263/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:48900/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:46806/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:46492/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:41732/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:39252/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:38271/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:36612/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:31930/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:31550/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:2484/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:2471/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:23819/1‑48 (MQ=255) aTGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGAAAATGCCg  >  1:21007/1‑48 (MQ=255)                                        |         ATGCCAGTATTACCGGCAGAGAGTGAGTAAATTTGCGGGGAAATGCCG  >  W3110S.gb/2716070‑2716117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑