Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,750,014	T→C	S176S (TCT→TCC)	yfjB →	NAD kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,750,014	0	T	C	100.0%	126.9 / NA	68	S176S (TCT→TCC)	yfjB	NAD kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/68);  total (0/68)

ATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGC  >  W3110S.gb/2749991‑2750052                        |                                       aTCTTTGCGTTTTCGGAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:61334/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:53521/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:64622/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:63715/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:63556/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:6348/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:60181/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:55110/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:54077/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:66488/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:52579/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:51476/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:50741/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:50480/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:49303/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:48944/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:1251/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:80446/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:88308/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:87776/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:87772/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:85633/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:83493/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:82974/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:82197/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:81807/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:4826/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:78091/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:77361/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:75146/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:7327/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:70458/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:70338/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:69123/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:29977/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:13783/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:15249/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:16365/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:18444/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:22422/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:22703/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:24119/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:2713/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:27707/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:2774/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:27845/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:29371/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:29501/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:47608/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:31449/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:37206/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:45048/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:39318/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:41259/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:34238/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:43199/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:44004/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:44242/62‑1 (MQ=255) aTCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:44917/62‑1 (MQ=255) aTCTTTGCGGTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:15724/62‑1 (MQ=255)  tCTTTGCGTTTTCGTAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:36784/61‑1 (MQ=255)  tCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:41916/61‑1 (MQ=255)  tCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:45197/61‑1 (MQ=255)  tCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:16069/61‑1 (MQ=255)  tCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:60085/61‑1 (MQ=255)  tCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:38459/61‑1 (MQ=255)  tCTTTGCGTTTTCGCAGCGTTCCGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:34800/61‑1 (MQ=255)  tCTTTGCGTTTTCGCAGCGTTACGATGGACTGATTATTTCGACGCCAACAGGCTCCACCGc  <  1:28662/61‑1 (MQ=255)                        |                                       ATCTTTGCGTTTTCTCAGCGATCTGATGGACTAATTATTTCGACGCCAACAGGCTCCACCGC  >  W3110S.gb/2749991‑2750052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑