Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,788,832	T→C	F65F (TTT→TTC)	ygaF →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,788,832	0	T	C	100.0%	103.3 / NA	56	F65F (TTT→TTC)	ygaF	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/56);  total (0/56)

CAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAC  >  W3110S.gb/2788814‑2788877                   |                                              cAGCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGC‑‑CCAAAGCCTTTTGCGATCAAAAc  <  1:75063/62‑1 (MQ=255)   gCCTGAAGGTGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:82221/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCTATCAAAAc  <  1:20599/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCTATCAAAAc  <  1:79377/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:77143/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:10045/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:55625/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:59843/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:62642/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:62909/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:63068/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:65905/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:67601/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:68252/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:68577/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:69832/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:73893/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:54410/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:776/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:80415/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:80458/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:8134/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:81771/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:82270/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:83181/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:84019/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:86104/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:86225/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:52460/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:10442/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:1349/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:17016/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:19955/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:2002/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:21728/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:24995/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:26338/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:27223/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:2950/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:32985/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:33218/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:33486/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:33855/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:34098/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:36666/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:38108/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:39890/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:40618/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:4438/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:44413/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:4657/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:46616/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:48541/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:54/62‑1 (MQ=255)   gCCTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACAAAAGCCTTTTGCGATCAAAAc  <  1:76522/62‑1 (MQ=255)    ccTGAAGGCGCAGTTCTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAc  <  1:58000/61‑1 (MQ=255)                   |                                              CAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAC  >  W3110S.gb/2788814‑2788877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑