Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,794,860	G→A	E177E (GAG→GAA)	csiR →	DNA‑binding transcriptional dual regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,794,860	0	G	A	100.0%	35.3 / NA	21	E177E (GAG→GAA)	csiR	DNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/21);  total (0/21)

AATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAT  >  W3110S.gb/2794851‑2794911          |                                                    aaTGCTGGAAGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAt  <  1:58603/61‑1 (MQ=255) aaTGCTGGAAGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAt  <  1:9569/61‑1 (MQ=255) aaTGCTGGAAGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAt  <  1:84312/61‑1 (MQ=255) aaTGCTGGAAGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAt  <  1:80/61‑1 (MQ=255) aaTGCTGGAAGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAt  <  1:7188/61‑1 (MQ=255) aaTGCTGGAAGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAt  <  1:68110/61‑1 (MQ=255) aaTGCTGGAAGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAt  <  1:63685/61‑1 (MQ=255) aaTGCTGGAAGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAt  <  1:62740/61‑1 (MQ=255) aaTGCTGGAAGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAt  <  1:61039/61‑1 (MQ=255) aaTGCTGGAAGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAt  <  1:60639/61‑1 (MQ=255) aaTGCTGGAAGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAt  <  1:6044/61‑1 (MQ=255) aaTGCTGGAAGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAt  <  1:11701/61‑1 (MQ=255) aaTGCTGGAAGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAt  <  1:58381/61‑1 (MQ=255) aaTGCTGGAAGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAt  <  1:5598/61‑1 (MQ=255) aaTGCTGGAAGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAt  <  1:4809/61‑1 (MQ=255) aaTGCTGGAAGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAt  <  1:45679/61‑1 (MQ=255) aaTGCTGGAAGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAt  <  1:44986/61‑1 (MQ=255) aaTGCTGGAAGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAt  <  1:37026/61‑1 (MQ=255) aaTGCTGGAAGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAt  <  1:35118/61‑1 (MQ=255) aaTGCTGGAAGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAt  <  1:18645/61‑1 (MQ=255)  aTGCTGGAAGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAt  <  1:39168/60‑1 (MQ=255)          |                                                    AATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAT  >  W3110S.gb/2794851‑2794911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑