Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	307,708	T→C	S283S (TCA→TCG)	yagX ←	predicted aromatic compound dioxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	307,708	0	T	C	100.0%	17.8 / NA	12	S283S (TCA→TCG)	yagX	predicted aromatic compound dioxygenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/0);  total (12/0)

GCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGG  >  W3110S.gb/307672‑307733                                     |                          gcccgccGCCGGTAAAAAGGCGTTCACTGGCGTGGCCGATTGGCTGCTGTCGAAGAtggtgg  >  1:74137/1‑62 (MQ=255) gcccgccGCCGGTAAAAAGGCGATCACTGGCGTGGCCGATTGGCTGCTGTCGAAGAtggtgg  >  1:24255/1‑62 (MQ=255) gcccgccGCCGGTAAAAAGGCGATCACTGGCGTGGCCGATTGGCTGCTGTCGAAGAtggtgg  >  1:2791/1‑62 (MQ=255) gcccgccGCCGGTAAAAAGGCGATCACTGGCGTGGCCGATTGGCTGCTGTCGAAGAtggtgg  >  1:28336/1‑62 (MQ=255) gcccgccGCCGGTAAAAAGGCGATCACTGGCGTGGCCGATTGGCTGCTGTCGAAGAtggtgg  >  1:36516/1‑62 (MQ=255) gcccgccGCCGGTAAAAAGGCGATCACTGGCGTGGCCGATTGGCTGCTGTCGAAGAtggtgg  >  1:36745/1‑62 (MQ=255) gcccgccGCCGGTAAAAAGGCGATCACTGGCGTGGCCGATTGGCTGCTGTCGAAGAtggtgg  >  1:48627/1‑62 (MQ=255) gcccgccGCCGGTAAAAAGGCGATCACTGGCGTGGCCGATTGGCTGCTGTCGAAGAtggtgg  >  1:62528/1‑62 (MQ=255) gcccgccGCCGGTAAAAAGGCGATCACTGGCGTGGCCGATTGGCTGCTGTCGAAGAtggtgg  >  1:78741/1‑62 (MQ=255) gcccgccGCCGGTAAAAAGGCGATCACTGGCGTGGCCGATTGGCTGCTGTCGAAGAtggtgg  >  1:79374/1‑62 (MQ=255) gcccgccGCCGGTAAAAAGGCGATCACTGGCGTGGCCGATTGGCTGCTGTCGAAGAtggtgg  >  1:84755/1‑62 (MQ=255) gcccgccGCCGGTAAAAAGGCGATCACTGGCGTGGCCGATTGGCTGCTGTCGAAGAtggtg   >  1:52589/1‑61 (MQ=255)                                     |                          GCCCGCCGCCGGTAAAAAGGCGATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGG  >  W3110S.gb/307672‑307733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑