Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,811,418	C→T	S49S (AGC→AGT)	emrB →	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,811,418	0	C	T	100.0%	75.3 / NA	42	S49S (AGC→AGT)	emrB	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (42/0);  total (42/0)

GAATCTGGGCTCATCGCTCAGCCAGGGAACGTGGGTAATCAC  >  W3110S.gb/2811397‑2811438                      |                     gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGAt     >  1:15311/1‑39 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCa   >  1:37925/1‑41 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCa   >  1:33435/1‑41 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:6997/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:60278/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:60463/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:60752/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:60870/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:61271/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:62158/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:66808/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:6693/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:6804/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:56476/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:7097/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:71138/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:75955/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:80673/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:80820/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:81194/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:82455/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:8600/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:9485/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:29722/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:11658/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:11684/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:16119/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:18660/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:21945/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:22148/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:23636/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:23959/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:28359/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:58741/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:40834/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:42301/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:50641/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:51160/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:54239/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:55484/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:55953/1‑42 (MQ=37) gAATCTGGGCTCATCACTCAGTCAGGGAACGTGGGTGATCAc  >  1:10258/1‑42 (MQ=37)                      |                     GAATCTGGGCTCATCGCTCAGCCAGGGAACGTGGGTAATCAC  >  W3110S.gb/2811397‑2811438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑