Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,815,989	T→C	A57A (GCA→GCG)	yqaB ←	predicted hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,815,989	0	T	C	100.0%	72.0 / NA	40	A57A (GCA→GCG)	yqaB	predicted hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/40);  total (0/40)

ATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCG  >  W3110S.gb/2815965‑2816026                         |                                      agcGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:1804/60‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:68970/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:14508/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:45457/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:46866/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:47950/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:48451/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:56907/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:63672/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:65196/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:66115/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:43569/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:70828/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:74236/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:74397/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:74663/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:76037/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:77995/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:87678/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:41098/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:16377/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:16726/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:19211/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:205/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:2098/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:21185/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:23253/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:24981/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:2874/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:3103/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:3219/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:38723/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:38850/62‑1 (MQ=255) aTCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:43081/62‑1 (MQ=255)  tCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:28027/61‑1 (MQ=255)  tCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:66668/61‑1 (MQ=255)  tCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:52173/61‑1 (MQ=255)  tCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:22107/61‑1 (MQ=255)  tCGGCCTGATTCAGCTCAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:46560/61‑1 (MQ=255)                  cAATAATCGCCTGAGCAATACGCCAGGTGGGCGAGCCATTAAgcg  <  1:30606/45‑1 (MQ=38)                         |                                      ATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCG  >  W3110S.gb/2815965‑2816026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑