Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,844,165	C→T	A321A (GCG→GCA)	hycE ←	hydrogenase 3, large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,844,165	0	C	T	100.0%	143.2 / NA	80	A321A (GCG→GCA)	hycE	hydrogenase 3, large subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/80);  total (0/80)

GGTTTTACGCGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGGAAGA  >  W3110S.gb/2844156‑2844217          |                                                     ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATTGAGGTTTCACGCACGCGgaaga  <  1:4519/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:5215/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:62336/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:62123/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:61868/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:60275/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:59495/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:58860/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:58143/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:55497/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:52734/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:63352/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:51864/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:51748/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:50907/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:50813/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:50647/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:49986/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:4934/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:46789/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:76322/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:9127/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:85647/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:84111/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:79330/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:7918/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:78085/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:77947/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:77843/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:76747/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:46681/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:74803/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:72284/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:72096/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:71509/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:71459/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:70826/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:7015/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:69400/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:63572/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:19158/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:10644/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:25979/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:25744/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:25403/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:24464/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:23397/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:22337/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:20962/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:19248/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:26109/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:18979/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:18943/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:18397/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:18371/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:17764/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:17047/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:15489/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:15290/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:11300/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:39801/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:46626/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:46435/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:46398/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:44023/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:4332/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:43073/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:41598/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:40927/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:26664/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:39476/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:39421/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:39411/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:36099/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:35617/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:33498/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:29495/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:28201/62‑1 (MQ=255) ggTTTTACGTGCACCGGTAAGGATCTCTGCCATCTTCATGGAGGTTTCACGCACGCGgaaga  <  1:79235/62‑1 (MQ=255)     ttACGTGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGgaaga  <  1:28831/58‑1 (MQ=255)          |                                                     GGTTTTACGCGCACCGGTAAGGATCTCTGCCATTTTCATGGAGGTTTCACGCACGCGGAAGA  >  W3110S.gb/2844156‑2844217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑