Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,855,339	G→T	Q42K (CAA→AAA)	ygbA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,855,339	0	G	T	100.0%	86.9 / NA	48	Q42K (CAA→AAA)	ygbA	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (48/0);  total (48/0)

TCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAAC  >  W3110S.gb/2855305‑2855348                                   |          tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:68142/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:51844/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:54774/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:57772/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:59266/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:59526/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:59791/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:61205/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:63996/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:65567/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:6730/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:67308/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:10590/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:69308/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:74910/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:75559/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:76964/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:77170/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:77511/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:77519/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:7813/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:81313/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:82203/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:83718/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:50251/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:10132/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:10782/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:12674/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:21785/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:22543/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:25334/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:29900/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:30172/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:34158/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:35091/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:35140/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:35682/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:36408/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:37998/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:40506/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:42802/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:45537/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:49227/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaagc  >  1:49274/1‑42 (MQ=38) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaag   >  1:34589/1‑42 (MQ=255) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaag   >  1:40909/1‑42 (MQ=255) tctTCGCCGAACACACATTTATCCAGCCGCTTTTTCGCGTaag   >  1:51321/1‑42 (MQ=255) tctTCGCCGAACACACATTTAACCAGCCGCTTTTTCGCGTaagc  >  1:84850/1‑42 (MQ=38)                                   |          TCTTCGCCGAACACACATTTATCCAGCCGCTTTTGCGCGTAAAC  >  W3110S.gb/2855305‑2855348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑