Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	309,203	A→T	S16S (TCT→TCA)	yagY ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	309,203	0	A	T	100.0%	71.2 / NA	41	S16S (TCT→TCA)	yagY	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (0/41);  total (0/41)

CGGAGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGT  >  W3110S.gb/309200‑309261    |                                                           cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:67830/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:41649/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:43405/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:4891/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:53335/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:54185/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:54968/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:57075/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:60784/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:65035/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:65281/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:41476/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:75723/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:79143/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:79783/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:80273/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:81466/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:83363/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:86553/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:86716/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:9183/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:3241/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:1260/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:15174/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:16116/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:18730/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:21497/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:25007/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:26746/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:26747/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:27615/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:10076/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:32643/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:32874/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:33265/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:34032/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:35811/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:37464/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:39529/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:40505/62‑1 (MQ=255) cGGTGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGt  <  1:40764/62‑1 (MQ=255)    |                                                           CGGAGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGT  >  W3110S.gb/309200‑309261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑