Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,027,426 G→T T81A (ACC→GCA)  ygfS ← predicted oxidoreductase, 4Fe‑4S ferredoxin‑type subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,027,4260GT100.0% 141.5 / NA 78T81A (ACC→GCA) ygfSpredicted oxidoreductase, 4Fe‑4S ferredoxin‑type subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (0/78);  total (0/78)

ACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGC  >  W3110S.gb/3027414‑3027475
            |                                                 
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:60215/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:6736/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:67190/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:65265/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:64316/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:63826/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:62587/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:6251/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:62146/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:60725/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:68264/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:57146/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:56807/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:56545/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:55938/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:55847/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:55486/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:53157/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:53056/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:51519/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:77233/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:9920/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:87650/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:85105/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:82849/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:81872/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:81075/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:80974/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:79837/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:78947/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:51368/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:75735/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:75680/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:73981/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:73524/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:72961/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:69985/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:69802/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:68968/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:209/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:10949/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:27528/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:26682/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:26253/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:25399/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:24881/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:23720/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:2246/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:21699/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:28107/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:18806/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:17952/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:15332/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:14454/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:14055/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:13433/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:11820/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:11153/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:4199/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:50318/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:4996/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:49944/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:48959/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:47999/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:4684/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:45643/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:28445/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:41863/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:40849/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:38211/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:35590/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:33672/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:32552/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:31531/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:30753/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:29546/62‑1 (MQ=255)
aCGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGGCAGCGCCCCCACGGGACAAgcgc  <  1:17723/62‑1 (MQ=255)
 cGGGCAGAATTTGCCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGACAAgcgc  <  1:14597/61‑1 (MQ=255)
            |                                                 
ACGGGCAGAATTGGTCTGCACCACCTGCTCGCCCATCGTCAGCGCCCCCACGGGGCAAGCGC  >  W3110S.gb/3027414‑3027475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑