Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,045,313	G→A	R795R (CGC→CGT)	gcvP ←	glycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,045,313	0	G	A	100.0%	118.1 / NA	64	R795R (CGC→CGT)	gcvP	glycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/64);  total (0/64)

CGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGTTGGCGTTGAGGATTGCCACCTGGCTTG  >  W3110S.gb/3045295‑3045356                   |                                            cGGGAAGGCATCCTGCAGACGGGTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:48222/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:70330/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:51757/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:53563/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:53764/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:54278/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:54534/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:55053/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:5558/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:56947/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:5733/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:59762/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:61396/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:62231/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:64255/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:66342/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:12096/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:71819/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:77073/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:78250/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:78321/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:80033/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:80393/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:81238/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:82760/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:83848/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:85242/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:85671/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:86358/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:8675/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:86914/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:87656/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:49238/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:10744/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:1683/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:18427/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:19599/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:22137/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:23524/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:23824/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:25348/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:25544/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:26857/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:27163/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:28744/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:29880/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:31499/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:32629/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:32638/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:33269/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:3392/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:34194/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:34612/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:36296/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:37954/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:42587/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:43922/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:46001/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:47273/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:47670/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:49173/62‑1 (MQ=255) cGGGAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:51472/62‑1 (MQ=255) cGGGAAGGCATCCTGAAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:46878/62‑1 (MQ=255)   ggAAGGCATCCTGCAGACGGCTGGCAATATAGTTGGCGTTAAGAATTGCCACCTGgcttg  <  1:88151/60‑1 (MQ=255)                   |                                            CGGGAAGGCATCCTGCAGGCGGCTGGCAATATAGTTGGCGTTGAGGATTGCCACCTGGCTTG  >  W3110S.gb/3045295‑3045356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑